Hi, as i said in my other message to the original post, you may have realised that:
once properly tuned,
* your 6th string (E) when played at the 5th fret will sound the same as the next string down (the A)
* Your 5th string (A) when played at the 5th fret will sound the same as the next string down (the D)
* Your 4th string (D) when played at the 5th fret will sound the same as the next string down (the G)
*your 3rd string (G) when played at the 4th fret will sound the same as the next string down (the B)
* your 2nd string (B) when played at the 5th fret will sound the same as the next and final string down (the E)
Alternatively, i could send you (on friday) a diagram of 'bar theory,' which shows where the different, and the same notes can be found on the neck of the guitar.
If you are a relatively new player, tuning by ear may just be something that will take time and experience (you will need to take time to know what that E string sounds like by memory.
but remember, some professional guitarist always rely on tuners. not everyone can do this. it's just a bonus if you do.
i hope this helps you
chris